libretro/slang-shaders

Name: slang-shaders

Owner: libretro

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Created: 2016-07-12 20:18:57.0

Updated: 2018-05-09 23:20:18.0

Pushed: 2018-05-02 19:26:35.0

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Language: C

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README

Vulkan GLSL RetroArch shader system

This document is a draft of RetroArch's new GPU shader system. It will outline the features in the new shader subsystem and describe details for how it will work in practice.

In addition this document will contain various musings on why certain design choices are made and which compromised have been made to arrive at the conclusion. This is mostly for discussing and deliberation while the new system is under development.

Introduction
Target shader languages

RetroArch is still expected to run on GLES2 and GL2 systems. GL2 is mostly not relevant any longer, but GLES2 is certainly a very relevant platform still and having GLES2 compatibility makes GL2 very easy. We therefore want to avoid speccing out a design which deliberately ruins GLES2 compatibility.

However, we also do not want to artificially limit ourselves to shader features which are only available in GLES2. There are many shader builtins for example which only work in GLES3/GL3 and we should not hold back support in these cases. When we want to consider GLES2 compat we should not spec out high level features which do not make much sense in the context of GLES2.

Why a new spec?

The current shader subsystem in RetroArch is quite mature with a large body of shaders written for it. While it has served us well, it is not forward-compatible.

The current state of writing high-level shading languages that work “everywhere” is very challenging. There was no good ready-made solution for this. Up until now, we have relied on nVidia Cg to serve as a basic foundation for shaders, but Cg has been discontinued for years and is closed source. This is very problematic since Cg is not a forward compatible platform. It has many warts which are heavily tied in to legacy APIs and systems. For this reason, we cannot use Cg for newer APIs such as Vulkan and potentially D3D12 and Metal.

Cg cross compilation to GLSL is barely working and it is horribly unmaintainable with several unfixable issues. The output is so horribly mangled and unoptimized that it is clearly not the approach we should be taking. We also cannot do the Cg transform in runtime on mobile due to lack of open source Cg runtime, so there's that as well.

Another alternative is to write straight-up GLSL, but this too has some severe problems. All the different GL versions and GLSL variants are different enough that it becomes painful to write portable GLSL code that works without modification. Examples include:

The problem really is that GLSL shaders are dependent on the runtime GL version, which makes it very annoying and hard to test all shader variants.

We do not want to litter every shader with heaps of #ifdefs everywhere to combat this problem. We also want to avoid having to write pseudo-GLSL with some text based replacement behind the scenes.

Vulkan GLSL as the portable solution

Fortunately, there is now a forward looking and promising solution to our problems. Vulkan GLSL is a GLSL dialect designed for Vulkan and SPIR-V intermediate representation. The good part is that we can use whatever GLSL version we want when writing shaders, as it is decoupled from the GL runtime.

In runtime, we can have a vendor-neutral mature compiler, https://github.com/KhronosGroup/glslang which compiles our Vulkan GLSL to SPIR-V. Using https://github.com/KhronosGroup/SPIRV-Cross, we can then do reflection on the SPIR-V binary to deduce our filter chain layout. We can also disassemble back to our desired GLSL dialect in the GL backend based on which GL version we're running, which effectively means we can completely sidestep all our current problems with a pure GLSL based shading system.

Another upside of this is that we no longer have to deal with vendor-specific quirks in the GLSL frontend. A common problem when people write for nVidia is that people mistakingly use float2/float3/float4 types from Cg/HLSL, which is supported as an extension in their GLSL frontend.

Why not SPIR-V directly?

This was considered, but there are several convenience problems with having a shading spec around pure SPIR-V. The first problem is metadata. In GLSL, we can quite easily extend with custom #pragmas or similar, but there is no trivial way to do this in SPIR-V outside writing custom tools to emit special metadata as debug information or similar with OpSource.

We could also have this metadata outside in a separate file, but juggling more files means more churn, which we should try to avoid. The other problem is convenience. If RetroArch only accepts SPIR-V, we would need an explicit build step outside RetroArch first before we could test a shader. This gets very annoying during shader development, so it is clear that we need to support GLSL anyways, making SPIR-V support kinda redundant.

The main argument for supporting SPIR-V would be to allow new shading languages to be used. This is a reasonable thing to consider, which is why the goal is to not design ourselves into a corner where it's only Vulkan GLSL that can possibly work down the line. We are open to the idea that new shading languages that target SPIR-V will emerge.

Warts in old shader system

While the old shader system is functional it has some severe warts which have accumulated over time. In hindsight, some of the early design decisions were misguided and need to be properly fixed.

Forced POT with padding

This is arguably the largest wart of them all. The original reason behind this design decision was caused by a misguided effort to combat FP precision issues with texture sampling. The idea at the time was to avoid cases where nearest neighbor sampling at texel edges would cause artifacts. This is a typical case when textures are scaled with non-integer factors. However, the problem to begin with is naive nearest neighbor and non-integer scaling factors, and not FP precision. It was pure luck that POT tended to give better results with broken shaders, but we should not make this mistake again. POT padding has some severe issues which are not just cleanliness related either.

Technically, GLES2 doesn't require non-POT support, but in practice, all GPUs support this.

No proper UV wrapping

Since the texture “ends” at UV coords < 1.0, we cannot properly use sampler wrapping modes. We can only fake CLAMP_TO_BORDER by padding with black color, but this filtering mode is not available by default in GLES2 and even GLES3! CLAMP_TO_BORDER isn't necessarily what we want either. CLAMP_TO_EDGE is usually a far more sane default.

Extra arguments for actual width vs. texture width

With normalized coordinates we need to think in both real resolution (e.g. 320x240) vs. POT padded resolutions (512x512) to deal with normalized UV coords. This complicates things massively and we were passing an insane amount of attributes and varyings to deal with this because the ratios between the two needn't be the same for two different textures.

Arbitrary limits

The way the old shader system deals with limits is quite naive. There is a hard limit of 8 when referencing other passes and older frames. There is no reason why we should have arbitrary limits like these. Part of the reason is C where dealing with dynamic memory is more painful than is should be so it was easier to take the lazy way out.

Tacked on format handling

In more complex shaders we need to consider more than just the plain RGBA8_UNORM format. The old shader system tacked on these things after the fact by adding booleans for SRGB and FP support, but this obviously doesn't scale. This point does get problematic since GLES2 has terrible support for render target formats, but we should allow complex shaders to use complex RT formats and rather just allow some shader presets to drop GLES2 compat.

PASS vs PASSPREV

Ugly. We do not need two ways to access previous passes, the actual solution is to have aliases for passes instead and access by name.

Inconsistencies in parameter passing

MVP matrices are passed in with weird conventions in the Cg spec, and its casing is weird. The source texture is passed with magic TEXUNIT0 semantic while other textures are passed via uniform struct members, etc. This is the result of tacking on feature support slowly over time without proper forethought.

High level Overview

The RetroArch shader format outlines a filter chain/graph, a series of shader passes which operate on previously generated data to produce a final result. The goal is for every individual pass to access information from all previous shader passes, even across frames, easily.

The backbuffer is somewhat special since the resolution of it cannot be controlled by the shader. It can also not be fed back into the filter chain later because the frontend (here RetroArch) will render UI elements and such on top of the final pass output.

Let's first look at what we mean by filter chains and how far we can expand this idea.

Simplest filter chain

The simplest filter chain we can specify is a single pass.

ut) -> [ Shader Pass #0 ] -> (Backbuffer)

In this case there are no offscreen render targets necessary since our input is rendered directly to screen.

Multiple passes

A trivial extension is to keep our straight line view of the world where each pass looks at the previous output.

ut) -> [ Shader Pass #0 ] -> (Framebuffer) -> [ Shader Pass #1 ] -> (Backbuffer)

Framebuffer here might have a different resolution than both Input and Backbuffer. A very common scenario for this is separable filters where we first scale horizontally, then vertically.

Multiple passes and multiple inputs

There is no reason why we should restrict ourselves to a straight-line view.

 /------------------------------------------------\
/                                                  v
ut) -> [ Shader Pass #0 ] -> (Framebuffer #0) -> [ Shader Pass #1 ] -> (Backbuffer)

In this scenario, we have two inputs to shader pass #1, both the original, untouched input as well as the result of a pass in-between. All the inputs to a pass can have different resolutions. We have a way to query the resolution of individual textures to allow highly controlled sampling.

We are now at a point where we can express an arbitrarily complex filter graph, but we can do better. For certain effects, time (or rather, results from earlier frames) can be an important factor.

Multiple passes, multiple inputs, with history

We now extend our filter graph, where we also have access to information from earlier frames. Note that this is still a causal filter system.

e N:        (Input     N, Input N - 1, Input N - 2) -> [ Shader Pass #0 ] -> (Framebuffer     N, Framebuffer N - 1, Input N - 3) -> [ Shader Pass #1 ] -> (Backbuffer)
e N - 1:    (Input N - 1, Input N - 2, Input N - 3) -> [ Shader Pass #0 ] -> (Framebuffer N - 1, Framebuffer N - 2, Input N - 4) -> [ Shader Pass #1 ] -> (Backbuffer)
e N - 2:    (Input N - 2, Input N - 3, Input N - 4) -> [ Shader Pass #0 ] -> (Framebuffer N - 2, Framebuffer N - 3, Input N - 5) -> [ Shader Pass #1 ] -> (Backbuffer)

For framebuffers we can read the previous frame's framebuffer. We don't really need more than one frame of history since we have a feedback effect in place. Just like IIR filters, the “response” of such a feedback in the filter graph gives us essentially “infinite” history back in time, although it is mostly useful for long-lasting blurs and ghosting effects. Supporting more than one frame of feedback would also be extremely memory intensive since framebuffers tend to be much higher resolution than their input counterparts. One frame is also a nice “clean” limit. Once we go beyond just 1, the floodgate opens to arbitrary numbers, which we would want to avoid. It is also possible to fake as many feedback frames of history we want anyways, since we can copy a feedback frame to a separate pass anyways which effectively creates a “shift register” of feedback framebuffers in memory.

Input textures can have arbitrary number of textures as history (just limited by memory). They cannot feedback since the filter chain cannot render into it, so it effectively is finite response (FIR).

For the very first frames, frames with frame N < 0 are transparent black (all values 0).

No POT padding

No texture in the filter chain is padded at any time. It is possible for resolutions in the filter chain to vary over time which is common with certain emulated systems. In this scenarios, the textures and framebuffers are simply resized appropriately. Older frames still keep their old resolution in the brief moment that the resolution is changing.

It is very important that shaders do not blindly sample with nearest filter with any scale factor. If naive nearest neighbor sampling is to be used, shaders must make sure that the filter chain is configured with integer scaling factors so that ambiguous texel-edge sampling is avoided.

Deduce shader inputs by reflection

We want to have as much useful information in the shader source as possible. We want to avoid having to explicitly write out metadata in shaders whereever we can. The biggest hurdle to overcome is how we describe our pipeline layout. The pipeline layout contains information about how we access resources such as uniforms and textures. There are three main types of inputs in this shader system.

Deduction by name

There are two main approaches to deduce what a sampler2D uniform wants to sample from. The first way is to explicitly state somewhere else what that particular sampler needs, e.g.

orm sampler2D geeWhatAmI;

etadata somewhere else
LER geeWhatAmI = Input[-2]; // Input frame from 2 frames ago

The other approach is to have built-in identifiers which correspond to certain textures.

ource here being defined as the texture from previous framebuffer pass or the input texture if this is the first pass in the chain.
orm sampler2D Source;

In SPIR-V, we can use OpName to describe these names, so we do not require the original Vulkan GLSL source to perform this reflection. We use this approach throughout the specification. An identifier is mapped to an internal meaning (semantic). The shader backend looks at these semantics and constructs a filter chain based on all shaders in the chain.

Identifiers can also have user defined meaning, either as an alias to existing identifiers or mapping to user defined parameters.

Combining vertex and fragment into a single shader file

One strength of Cg is its ability to contain multiple shader stages in the same .cg file. This is very convenient since we always want to consider vertex and fragment together. This is especially needed when trying to mix and match shaders in a GUI window for example. We don't want to require users to load first a vertex shader, then fragment manually.

GLSL however does not support this out of the box. This means we need to define a light-weight system for preprocessing one GLSL source file into multiple stages.

Should we make vertex optional?

In most cases, the vertex shader will remain the same. This leaves us with the option to provide a “default” vertex stage if the shader stage is not defined.

#include support

With complex filter chains there is a lot of oppurtunity to reuse code. We therefore want light support for the #include directive.

User parameter support

Since we already have a “preprocessor” of sorts, we can also trivially extend this idea with user parameters. In the shader source we can specify which uniform inputs are user controlled, GUI visible name, their effective range, etc.

Lookup textures

A handy feature to have is reading from lookup textures. We can specify that some sampler inputs are loaded from a PNG file on disk as a plain RGBA8 texture.

Do we want to support complex reinterpretation?

There could be valid use cases for supporting other formats than plain RGBA8_UNORM. SRGB and UINT might be valid cases as well and maybe even 2x16-bit, 1x32-bit integer formats.

Lookup buffers

Do we want to support lookup buffers as UBOs as well? This wouldn't be doable in GLES2, but it could be useful as a more modern feature. If the LUT is small enough, we could realize it via plain old uniforms as well perhaps.

This particular feature could be very interesting for generic polyphase lookup banks with different LUT files for different filters.

Vulkan GLSL specification

This part of the spec considers how Vulkan GLSL shaders are written. The frontend uses the glslang frontend to compile GLSL sources. This ensures that we do not end up with vendor-specific extensions. The #version string should be as recent as possible, e.g. #version 450 or #version 310 es. It is recommended to use 310 es since it allows mediump which can help on mobile. Note that after the Vulkan GLSL is turned into SPIR-V, the original #version string does not matter anymore. Also note that SPIR-V cannot be generated from legacy shader versions such as #version 100 (ES 2.0) or #version 120 (GL 2.1).

The frontend will use reflection on the resulting SPIR-V file in order to deduce what each element in the UBO or what each texture means. The main types of data passed to shaders are read-only and can be classified as:

Resource usage rules

Certain rules must be adhered to in order to make it easier for the frontend to dynamically set up bindings to resources.

Initial preprocess of slang files

The very first line of a .slang file must contain a #version statement.

The first process which takes place is dealing with #include statements. A slang file is preprocessed by scanning through the slang and resolving all #include statements. The include process does not consider any preprocessor defines or conditional expressions. The include path must always be relative, and it will be relative to the file path of the current file. Nested includes are allowed, but includes in a cycle are undefined as preprocessor guards are not considered.

E.g.:

lude "common.inc"

After includes have been resolved, the frontend scans through all lines of the shader and considers #pragma statements. These pragmas build up ancillary reflection information and otherwise meaningful metadata.

#pragma stage

This pragma controls which part of a .slang file are visible to certain shader stages. Currently, two variants of this pragma are supported:

If no #pragma stage has been encountered yet, lines of code in a shader belong to all shader stages. If a #pragma stage statement has been encountered, that stage is considered active, and the following lines of shader code will only be used when building source for that particular shader stage. A new #pragma stage can override which stage is active.

#pragma name

This pragma lets a shader set its identifier. This identifier can be used to create simple aliases for other passes.

E.g.:

gma name HorizontalPass
#pragma format

This pragma controls the format of the framebuffer which this shader will render to. The default render target format is R8G8B8A8_UNORM.

Supported render target formats are listed below. From a portability perspective, please be aware that GLES2 has abysmal render target format support, and GLES3/GL3 may have restricted floating point render target support.

If rendering to uint/int formats, make sure your fragment shader output target is uint/int.

8-bit 10-bit 16-bit 32-bit

E.g.:

gma format R16_SFLOAT
#pragma parameter

Shader parameters allow shaders to take user-defined inputs as uniform values. This makes shaders more configurable.

The format is:

gma parameter IDENTIFIER "DESCRIPTION" INITIAL MINIMUM MAXIMUM [STEP]

The step parameter is optional. INITIAL, MINIMUM and MAXIMUM are floating point values. IDENTIFIER is the meaningful string which is the name of the uniform which will be used in a UBO or push constant block. DESCRIPTION is a string which is human readable representation of IDENTIFIER.

E.g:

ut(push_constant) uniform Push {
loat DummyVariable;
gisters;
gma parameter DummyVariable "This is a dummy variable" 1.0 0.2 2.0 0.1
I/O interface variables

The slang shader spec specifies two vertex inputs and one fragment output. Varyings between vertex and fragment shaders are user-defined.

Vertex inputs

Two attributes are provided and must be present in a shader. It is only the layout(location = #N) which is actually significant. The particular names of input and output variables are ignored, but should be consistent for readability.

layout(location = 0) in vec4 Position;

This attribute is a 2D position in the form vec4(x, y, 0.0, 1.0);. Shaders should not try to extract meaning from the x, y. gl_Position must be assigned as:

osition = MVP * Position;
layout(location = 1) in vec2 TexCoord;

The texture coordinate is semantically such that (0.0, 0.0) is top-left and (1.0, 1.0) is bottom right. If TexCoord is passed to a varying unmodified, the interpolated varying will be uv = 0.5 / OutputSize when rendering the upper left pixel as expected and uv = 1.0 - 0.5 / OutputSize when rendering the bottom-right pixel.

Vertex/Fragment interface

Vertex outputs and fragment inputs link by location, and not name.

E.g.:

ertex
ut(location = 0) out vec4 varying;
ragment
ut(location = 0) in vec4 some_other_name;

will still link fine, although using same names are encouraged for readability.

Fragment outputs layout(location = 0) out vec4 FragColor;

Fragment shaders must have a single output to location = 0. Multiple render targets are not allowed. The type of the output depends on the render target format. int/uint type must be used if UINT/INT render target formats are used, otherwise float type.

Builtin variables
Builtin texture variables

The input of textures get their meaning from their name.

Builtin texture size uniform variables

If a member of a UBO or a push constant block is called ???Size# where ???# is the name of a texture variable, that member must be a vec4, which will receive these values:

It is valid to use a size variable without declaring the texture itself. This is useful for vertex shading. It is valid (although probably not useful) for a variable to be present in both a push constant block and a UBO block at the same time.

Builtin uniform variables

Other than uniforms related to textures, there are other special uniforms available. These builtin variables may be part of a UBO block and/or a push constant block.

Aliases

Aliases can give meaning to arbitrary names in a slang file. This is mostly relevant for LUT textures, shader parameters and accessing other passes by name.

If a shader pass has a #pragma name NAME associated with it, meaning is given to the shader:

Example slang shader
sion 450
50 or 310 es are recommended

ut(set = 0, binding = 0, std140) uniform UBO

at4 MVP;
ec4 SourceSize; // Not used here, but doesn't hurt
loat ColorMod;


gma name StockShader
gma format R8G8B8A8_UNORM
gma parameter ColorMod "Color intensity" 1.0 0.1 2.0 0.1

gma stage vertex
ut(location = 0) in vec4 Position;
ut(location = 1) in vec2 TexCoord;
ut(location = 0) out vec2 vTexCoord;
 main()

l_Position = MVP * Position;
TexCoord = TexCoord;


gma stage fragment
ut(location = 0) in vec2 vTexCoord;
ut(location = 0) out vec4 FragColor;
ut(binding = 1) uniform sampler2D Source;
 main()

ragColor = texture(Source, vTexCoord) * ColorMod;

Push constants vs uniform blocks

Push constants are fast-access uniform data which on some GPUs will improve performance over plain UBOs. It is encouraged to use push constant data as much as possible.

ut(push_constant) uniform Push

ec4 SourceSize;
ec4 FinalViewportSize;
gisters;

However, be aware that there is a limit to how large push constant blocks can be used. Vulkan puts a minimum required size of 128 bytes, which equals 8 vec4s. It is an error to use more than 128 bytes. If you're running out of space, you can move the MVP to a UBO instead, which frees up 64 bytes. Always prioritize push constants for data used in fragment shaders as there are many more fragment threads than vertex. Also note that like UBOs, the push constant space is shared across vertex and fragment.

If you need more than 8 vec4s, you can spill uniforms over to plain UBOs, but more than 8 vec4s should be quite rare in practice.

E.g.:

ut(binding = 0, std140) uniform UBO

at4 MVP; // Only used in vertex
ec4 SpilledUniform;
obal;

ut(push_constant) uniform Push

ec4 SourceSize;
ec4 BlurPassSize;
/ ...
gisters;
Samplers

Which samplers are used for textures are specified by the preset format. The sampler remains constant throughout the frame, there is currently no way to select samplers on a frame-by-frame basic. This is mostly to make it possible to use the spec in GLES2 as GLES2 has no concept of separate samplers and images.

sRGB

The input to the filter chain will not be of an sRGB format. This is due to many reasons, the main one being that it is very difficult for the frontend to get “free” passthrough of sRGB. It is possible to have a first pass which linearizes the input to a proper sRGB render target. In this way, custom gammas can be used as well.

Similarly, the final pass will not be an sRGB backbuffer for similar reasons.

Caveats
Frag Coord

TexCoord also replaces gl_FragCoord. Do not use gl_FragCoord as it doesn't consider the viewports correctly. If you need gl_FragCoord use vTexCoord * OutputSize.xy instead.

Derivatives

Be careful with derivatives of vTexCoord. The screen might have been rotated by the vertex shader, which will also rotate the derivatives, especially in the final pass which hits the backbuffer. However, derivatives are fortunately never really needed, since w = 1 (we render flat 2D quads), which means derivatives of varyings are constant. You can do some trivial replacements which will be faster and more robust.

(vTexCoord) = vec2(OutputSize.z, 0.0);
(vTexCoord) = vec2(0.0, OutputSize.w);
th(vTexCoord) = max(OutputSize.z, OutputSize.w);

To avoid issues with rotation or unexpected derivatives in case derivatives are really needed, off-screen passes will not have rotation and dFdx and dFdy will behave as expected.

Correctly sampling textures

A common mistake made by shaders is that they aren't careful enough about sampling textures correctly. There are three major cases to consider

Bilinear sampling

If bilinear is used, it is always safe to sample a texture.

Nearest, with integer scale

If the OutputSize / InputSize is integer, the interpolated vTexCoord will always fall inside the texel safely, so no special precautions have to be used. For very particular shaders which rely on nearest neighbor sampling, using integer scale to a framebuffer and upscaling that with more stable upscaling filters like bicubic for example is usually a great choice.

Nearest, with non-integer scale

Sometimes, it is necessary to upscale images to the backbuffer which have an arbitrary size. Bilinear is not always good enough here, so we must deal with a complicated case.

If we interpolate vTexCoord over a frame with non-integer scale, it is possible that we end up just between two texels. Nearest neighbor will have to find a texel which is nearest, but there is no clear “nearest” texel. In this scenario, we end up having lots of failure cases which are typically observed as weird glitches in the image which change based on the resolution.

To correctly sample nearest textures with non-integer scale, we must pre-quantize our texture coordinates. Here's a snippet which lets us safely sample a nearest filtered texture and emulate bilinear filtering.

ec2 uv = vTexCoord * global.SourceSize.xy - 0.5; // Shift by 0.5 since the texel sampling points are in the texel center.
ec2 a = fract(uv);
ec2 tex = (floor(uv) + 0.5) * global.SourceSize.zw; // Build a sampling point which is in the center of the texel.

/ Sample the bilinear footprint.
ec4 t0 = textureLodOffset(Source, tex, 0.0, ivec2(0, 0));
ec4 t1 = textureLodOffset(Source, tex, 0.0, ivec2(1, 0));
ec4 t2 = textureLodOffset(Source, tex, 0.0, ivec2(0, 1));
ec4 t3 = textureLodOffset(Source, tex, 0.0, ivec2(1, 1));

/ Bilinear filter.
ec4 result = mix(mix(t0, t1, a.x), mix(t2, t3, a.x), a.y);

The concept of splitting up the integer texel along with the fractional texel helps us safely do arbitrary non-integer scaling safely. The uv variable could also be passed pre-computed from vertex to avoid the extra computation in fragment.

Preset format (.slangp)

The present format is essentially unchanged from the old .cgp and .glslp, except the new preset format is called .slangp.

Porting guide from legacy Cg spec
Common functions
Types
Builtin uniforms and misc
Cg semantics

Output structs should be flattened into separate varyings.

E.g. instead of

ct VertexData

loat pos : POSITION;
loat4 tex0 : TEXCOORD0;
loat4 tex1 : TEXCOORD1;


 main_vertex(out VertexData vout)

out.pos = ...;
out.tex0 = ...;
out.tex1 = ...;


 main_fragment(in VertexData vout)

..

do this

gma stage vertex
ut(location = 0) out vec4 tex0;
ut(location = 1) out vec4 tex1;
 main()

l_Position = ...;
ex0 = ...;
ex1 = ...;


gma stage fragment
ut(location = 0) in vec4 tex0;
ut(location = 1) in vec4 tex1;
 main()


Instead of returning a float4 from main_fragment, have an output in fragment:

ut(location = 0) out vec4 FragColor;

This work is supported by the National Institutes of Health's National Center for Advancing Translational Sciences, Grant Number U24TR002306. This work is solely the responsibility of the creators and does not necessarily represent the official views of the National Institutes of Health.