SynBioDex/SEVA

Name: SEVA

Owner: Synthetic Biology Data Exchange Group

Description: SBOL representation of plasmids that conform to the Standard European Vector Architecture

Created: 2018-05-18 03:06:45.0

Updated: 2018-05-18 03:07:31.0

Pushed: 2018-05-18 03:07:29.0

Homepage: null

Size: 111

Language: Python

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README

SEVA in SBOL

The script seva.py constructs an SBOL representation of plasmids that conform to the Standard European Vector Architecture (SEVA). Custom cargos can be ``cloned'' into the SEVA multiple cloning site.

DEPENDENCIES

The seva.py script depends on pySBOL. For installation options, go to Installation Page.

EXAMPLE
eate a custom insert in the Cargo region
= Document()
 doc.componentDefinitions.create('insert')
= doc.sequences.create('insert')
elements = 'actg'
equence = seq
Cargo('my_cargo')
sert(cd, 'PstI', 'SpeI')  # insert the cargo between the PstI and SpeI restriction sites

ite the SEVA vector as SBOL
er_id = 1  # According to SEVA standard, the selection marker can be 1 - 6
in_id = 1  # The origin marker can be 1 - 9
SEVAVector('pSEVA11my_cargo', marker_id, origin_id, c)  # Writes the file pSEVAmy_cargo.xml

This work is supported by the National Institutes of Health's National Center for Advancing Translational Sciences, Grant Number U24TR002306. This work is solely the responsibility of the creators and does not necessarily represent the official views of the National Institutes of Health.